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  • Terry Branch posted an update 3 months, 2 weeks ago

    Doubly periodic (left) or fixed (correct) boundary conditions are imposed. Parameter values are listed in Table . (B) Comparison of P compartment locations and cell numbers for wt, endap and Title Loaded From File enCycE simulations where doubly periodic or fixed boundary conditions are imposed. Mean values from simulations are shown and error bars are normal deviations. Shaded areas mark the ranges of experimentally observed values and are added for reference and comparison with Figs , and S. (TIFF) S Fig. Compartment boundary line tension does not influence P compartment sizes and cell numbers. (A) Snapshots of a wt simulation in the final time point, once all cell divisions have occurred, exactly where either a higher (left) or low (correct) line tension, L, is imposed in the boundary between A and P compartments. Parameter values are listed in Table . Compartment boundary line tension promotes cell sorting and straightness of your boundary, but will not impact compartment sizes. (B) Comparison of P compartment areas and cell numbers for wt, endap and enCycE simulations where a high (left) or low (proper) compartment boundary line ten sion is imposed. Values for L at the compartment boundary are these given in (A). Imply values from simulations are shown and error bars are typical deviations. Shaded places markPLOS Computational Biology DOI:.journal.pcbi. December , Tissue Size Handle through Passive Mechanical Forcesthe ranges of experimentally observed values and are added for reference and comparison with Figs , and S. (TIFF) S Fig. Initial cell shapes do not substantially influence P compartment sizes and cell numbers. (A) Snapshots of a hexagonal (left) initial situation, and a sample random (right) initial situation, as described in S Text. The cells assigned for the posterior compartment occupy a comparable region in both photos. (B) Comparison of P compartment places and cell numbers for wt, endap and enCycE simulations where either a hexagonal or random initial condition (IC) was employed. Mean values from simulations are shown and error bars are normal deviations. Shaded locations mark the ranges of experimentally observed values and are added for reference and comparison with Figs , and S. Parameter values are listed in Table . (TIFF) S Fig. Influence of initial cell target area on P compartment size and cell numbers. (B) Comparison of P compartment locations and cell numbers for wt, endap and enCycE simulations exactly where either initial target areas As m or As m were employed. Mean values from simulations are shown and error bars are normal deviations. Shaded locations mark the ranges of experimentally observed values and are added for reference and comparison with Figs , and S. Parameter values are listed in Table . (TIFF) S Table. Occurrence of T swaps in simulations. For every single case of the passive mechanical model thought of in Fig , we present the imply number of T swaps across simulations. The indicated errors are regular deviations. (PDF)PAB and JJZ acknowledge the Notre Dame Integrated Imaging Facility to create experimental figures. JK acknowledges the use of the University of Oxford Advanced Analysis Computing facility in carrying out this operate.Author ContributionsConceived and developed the experimentsPAB JJZ. Performed the experimentsPAB. Analyzed the dataJK JJZ REB AGF. Contributed reagentsmaterialsanalysis toolsJJZ. Wrote the paperJK PAB JJZ REB AGF. Conceived and created the simulation studyJK JJZ REB AGF. Performed the simulation studyJK.